About the Webinar

Inverted terminal repeats (ITRs) of adeno-associated virus (AAV) play a key role in virus replication and encapsidation, and are also important in viral genome integration and excision from the host genome. However, due to their high GC content and secondary structures, AAV plasmid ITRs are frequently mutated during plasmid production in E. coli., reducing experiment reproducibility and causing delays if ITR mutations are left undetected. In this presentation, Dr. Elizabeth Louie from GENEWIZ will discuss our novel adeno-associated virus (AAV) plasmid DNA sequencing (RUO/GLP), preparation, and synthesis solutions for rAAV production while confirming, maintaining, and correcting inverted terminal repeat (ITR) regions. In addition, she will also talk about GENEWIZ’s proprietary Sanger & NGS viral genome sequencing confirmation solutions post-AAV virus packaging, including at the regulatory level, to optimize AAV production for gene therapy development.

About the Presenter



Elizabeth Louie is the supervisor of the Technical Applications team at GENEWIZ. She received her Ph.D. in Molecular and Cellular Pharmacology from Stony Brook University. First joining GENEWIZ in 2011, Elizabeth oversaw gene synthesis and molecular genetics projects, and helped the Project Management team with project design. She later helped expand and manage molecular biology and oligo synthesis operations at GENEWIZ Boston. In her current role, Elizabeth provides consultation to customers across all genomic solutions that GENEWIZ offers, including next generation sequencing, gene and DNA synthesis, and any custom molecular biology needs.

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