Reading Through the Inverted Terminal Repeats (ITRs) of Adeno-Associated Virus (AAV): Azenta's Proprietary Sanger Sequencing Method
About the Tech Note
The inverted terminal repeat (ITR) regions of adeno-associated virus genomes are important for rAAV production because of their key role in virus replication and encapsidation. These ITRs form highly-stable secondary structures that pose major obstacles for sequence verification using existing technologies. To alleviate this issue, Azenta has developed a new proprietary Sanger sequencing method that allows qualitative assessment of the integrity of these ITR regions
Azenta’s proprietary Sanger sequencing protocol is now GLP-compliant. For more information on our GLP AAV sequencing services please click here.