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AAV-ITR Sequencing Azenta GENEWIZ

TECH NOTE

Reading Through the Inverted Terminal Repeats (ITRs) of Adeno-Associated Virus (AAV): Azenta's Proprietary Sanger Sequencing Method

About the tech note

The inverted terminal repeat (ITR) regions of adeno-associated virus genomes are important for rAAV production because of their key role in virus replication and encapsidation. These ITRs form highly-stable secondary structures that pose major obstacles for sequence verification using existing technologies. To alleviate this issue, Azenta has developed a new proprietary Sanger sequencing method that allows qualitative assessment of the integrity of these ITR regions.

Azenta’s proprietary Sanger sequencing protocol is now GLP-compliant. For more information on our GLP AAV plasmid sequencing services please click here.

 

What you'll learn

  • Importance of ITR regions in rAAV production
  • Challenges of sequencing the AAV-ITR regions
  • How Azenta's direct Sanger Sequencing method can sequence the AAV-ITRS

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For technical support questions, please contact our Technical Support team

+1 (877) 436-3949 ext. 3350 | molgen@azenta.com