About the tech note

The inverted terminal repeat (ITR) regions of adeno-associated virus genomes are important for rAAV production because of their key role in virus replication and encapsidation. These ITRs form highly-stable secondary structures that pose major obstacles for sequence verification using existing technologies. To alleviate this issue, Azenta has developed a new proprietary Sanger sequencing method that allows qualitative assessment of the integrity of these ITR regions.

Azenta’s proprietary Sanger sequencing protocol is now GLP-compliant. For more information on our GLP AAV plasmid sequencing services please click here.

What you'll learn

• Importance of ITR regions in rAAV production
• Challenges of sequencing the AAV-ITR regions
• How Azenta's direct Sanger Sequencing method can sequence the AAV-ITRS